Selected Recent Publications

  1. Stöger R, Choi M, Begum K, Leeman G, Emes RD, Melamed P, Bentley GR. Childhood environment influences epigenetic age and methylation concordance of a CpG clock locus in British-Bangladeshi migrants. Epigenetics. 2022 Dec 10:1-10. doi: 10.1080/15592294.2022.2153511. Epub ahead of print. PMID: 36495138.
  2. Bar-Sadeh B, Amichai OE, Pnueli L, Begum K, Leeman G, Emes RD, Stöger R, Bentley GR, Melamed P. Epigenetic regulation of 5α reductase-1 underlies adaptive plasticity of reproductive function and pubertal timing. BMC Biol. 2022 Jan 7;20(1):11. doi: 10.1186/s12915-021-01219-6. PMID: 34996447; PMCID: PMC8742331.
  3. Khamis H, Rudnizky S, Melamed P, Kaplan A. Single molecule characterization of the binding kinetics of a transcription factor and its modulation by DNA sequence and methylation. Nucleic Acids Res. 2021 Nov 8;49(19):10975-10987. doi: 10.1093/nar/gkab843. PMID: 34606618; PMCID: PMC8565314.
  4. Bentley GR, Bogin BA, Bar-Sadeh B, Stöger R, Melamed P. Reply to: Timing of puberty – body size or reproductive optimization? Nat Rev Endocrinol. 2021 Sep;17(9):573-574. doi: 10.1038/s41574-021-00502-4. PMID: 33958767.
  5. Rudnizky S, Khamis H, Ginosar Y, Goren E, Melamed P, Kaplan A. Extended and dynamic linker histone-DNA Interactions control chromatosome compaction. Mol Cell. 2021 Aug 19;81(16):3410-3421.e4. doi: 10.1016/j.molcel.2021.06.006. Epub 2021 Jun 29. PMID: 34192510.
  6. Refael T, Melamed P. Enhancing Gonadotrope Gene Expression Through Regulatory lncRNAs. Endocrinology. 2021 Aug 1;162(8):bqab116. doi: 10.1210/endocr/bqab116. PMID: 34111287.
  7. Pnueli L, Shalev D, Refael T, David C, Boehm U, Melamed P. Proliferating primary pituitary cells as a model for studying regulation of gonadotrope chromatin and gene expression. Mol Cell Endocrinol. 2021 Aug 1;533:111349. doi: 10.1016/j.mce.2021.111349. Epub 2021 Jun 4. PMID: 34090968.
  8. Shalev D, Melamed P. The role of the hypothalamus and pituitary epigenomes in central activation of the reproductive axis at puberty. Mol Cell Endocrinol. 2020 Dec 1;518:111031
  9. Bar-Sadeh B, Rudnizky S, Pnueli L, Bentley GR, Stöger R, Kaplan A, Melamed P. Unravelling the role of epigenetics in reproductive adaptations to early-life environment. Nature Reviews Endocrinology. 2020 Sep;16(9):519-533.
  10. Rudnizky, S., Khamis, H., Malik, O., Melamed, P., & Kaplan, A. 2019. The base pair-scale diffusion of nucleosomes modulates binding of transcription factors. Proceedings of the National Academy of Sciences, 116(25), 12161-12166.‏
  11. Feldman, A., Saleh, A., Pnulei, L., Qiao, S., Shlomi, T., Boehm, U., Melamed, P. 2018. Sensitivity of pituitary gonadotropes to hyperglycemia leads to epigenetic aberrations and reduced follicle-stimulating hormone levels. FASEB J. 2019 Jan;33(1):1020-1032
  12. Melamed, P., Yosefzon, Y, David, C., Tsukerman, A., Pnueli, L. 2018. Tet enzymes, variants and differential effects on function. Front Cell Dev Biol. 6:22.
  13. Rudnizky, S., Khamis, H., Malik, O., Squires, A.H., Meller, A., Melamed, P., Kaplan, A. 2018. Single-molecule DNA unzipping reveals asymmetric modulation of the transcription factor Egr-1 by its binding site sequence and context. Nucleic Acids Res.  46:1513-1524.
  14. Melamed, P., Haj, M., Yosefzon, Y., Rudnizky, S., Wijeweera, A., Pnueli, L., Kaplan, A. 2018. Mutifaceted targeting of the chromatin mediates GnRH effects on gene expression in the gonadotrope. Front Endocrinol (Lausanne) 9:58.
  15. Yosefzon Y, David C, Tsukerman A, Pnueli L, Qiao S, Boehm U, Melamed P. 2017. An epigenetic switch repressing Tet1 in gonadotropes activates the reproductive axis. Proc. Natl. Acad. Sci. USA. 114:10131-10136
  16. Rudnizky S, Malik O, Bavly A, Pnueli L, Melamed P, Kaplan A. 2017. Nucleosome mobility and the regulation of gene expression: Insights from single-molecule studies. Protein Sci. 26:1266-1277.
  17. Haj, M., Wijeweera, A., Rudnizky, S., Taunton, J., Pnueli, L., Melamed, P. 2017. Mitogen- and stress-activated protein kinase 1 is required for gonadotropin-releasing hormone-mediated activation of gonadotropin α-subunit expression. J. Biol. Chem. 292:20720-20731
  18. Rahamim-Ben Navi, L., Tsukerman, A., Feldman, A., Melamed, P., Tomić, M., Stojilkovic, S.S., Boehm, U., Seger, R., Naor, Z. 2017. GnRH induces bleb formation in gonadotrope cells, involving recruitment of members of a GnRH receptor-associated signalosome to the blebs. Frontiers Endocrinology 8:113.
  19. Rudnizky S, Bavly A, Malik O, Pnueli L, Melamed P, Kaplan A. 2016. H2A.Z controls the stability and mobility of nucleosomes to regulate expression of the LH genes. Nat Commun. Sep 22;7:12958
  20. Melamed P, Yosefzon Y, Rudnizky S, Pnueli L. 2016. Transcriptional enhancers: Transcription, function and flexibility.Transcription. 7:26-31
  21. Pnueli, L., Yosefzon, Y., Rudnizky, S. and Melamed P. 2015. RNA transcribed from a distal enhancer is required for histone modifications at the promoter of the gonadotropin α-subunit gene. Proc Natl. Acad. Sci. USA. 2015 112:4369-74
  22. Wijeweera, A., Haj, M., Feldman, A., Pnueli L., Luo, Z. and Melamed P. 2015. Gonadotropin gene transcription is activated by Menin-mediated effects on the chromatin. Biochimica et Biophysica Acta 1849:328-41.
  23. Savulescu, D., Feng, J, Ping Y.S., Mai O., Boehm U., He B., O’Malley B.W., Melamed P. 2013. Gonadotropin-releasing hormone-regulated prohibitin mediates apoptosis of the gonadotrope cells. Mol. Endocrinol. 27:1856-70.
  24. Melamed, P., Savulescu, D., Lim, S., Wijeweera, A., Luo, Z.J., Luo, M., Pnueli, L. 2012. Gonadotropin releasing hormone (GnRH)-signalling downstream of calmodulin. J. Neuroendocrinol. 24:1463-75.
  25. Pnueli, L., Luo, M., Wang, S., Naor, Z. and Melamed, P. 2011. Calcineurin regulates expression of both subunits of the Follicle-Stimulating Hormone through distinct mechanisms. Mol. Cell. Biol. 31:5023-36.
  26. Melamed, P. 2010. Hormonal signaling to FSHβ gene expression. Mol. Cell. Endocrinol. 314:204-12.
  27. Luo, Z.J., Wijeweera, A., Oh, Y.Z., Liou, Y.C. and Melamed, P. 2010. Pin1 regulates gonadotropin β-subunit gene transcription through facilitating the phosphorylation-dependent ubiquitination of SF-1. Mol. Cell. Biol. 30:745-63.
  28. Dobkin-Bekman, M., Naidich, M., Rahamim, L., Przedecki, F., Almog, T., Lim, S., Melamed, P., Liu, P., Wohland, T., Yao, Z., Seger, R. and Naor Z. 2009. A preformed signaling complex mediates GnRH activated ERK phosphorylation of paxillin and FAK at focal adhesions in LβT2 gonadotrope cells. Mol. Endocrinol. 23:1850-64.
  29. Lim, S., Pnueli, L., Tan, J.H., Naor Z., Rajagopal, G. and Melamed P. 2009. Negative feedback governs gonadotrope frequency-decoding of gonadotropin releasing hormone pulse-frequency PLoS One 4:e7244
  30. Wang, S., Zhu, Y. and Melamed, P. 2009. The molecular regulation of Chinook salmon gonadotropin β-subunit gene transcription. Gen. Comp. Endocrinol. 161:34-41.
  31. Feng, J. Lawson, M.A. and Melamed, P. 2008. A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing. Biol. Reprod. 79:546-61.
  32. Melamed, P. 2008. The role of histone deacetylases in expression of gonadotropin subunit genes. Trends Endocrinol. Metab. 19:25-31.
  33. Lim, S., Luo, M., Koh, M., Yang, M., Abdul Kadir, M.N., Tan, J.H., Ye, Z., Wang, W. and Melamed, P. 2007. Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin β-subunit genes in immature gonadotropes, and their actions are overcome by GnRH. Mol. Cell. Biol. 27:4105-20.
  34. Melamed, P. Abdul Kadir, M.N., Wijeweera, A. and Seah, S. 2006. Transcription of gonadotropin β subunit genes involves cross-talk between the transcription factors and co-regulators that mediate actions of the regulatory hormones. Mol. Cell. Endocrinol. 252:167-83.
  35. Melamed, P., Zhu, Y., Tan, S.H., Xie, M. and Koh. M. 2006. Gonadotropin releasing hormone activation of c-jun, but not Egr-1, stimulates transcription of a luteinizing hormone β subunit gene. Endocrinology 147:3598-605.
  36. Luo, M., Koh, M., Feng, J., Wu, Q. and Melamed, P. 2005. Cross-talk in hormonally-regulated gene transcription through induction of estrogen receptor ubiquitylation. Mol. Cell. Biol. 25:7386-98.